Summary of BTT306 TECHNIQUES IN BIOTECHNOLOGY

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BTT306 TECHNIQUES IN BIOTECHNOLOGY

Lecturer: DR. CHEW BEE LYNN
Lecturer: PROFESOR MADYA DR. AHMAD RAMLI BIN MOHD YAHYA
Lecturer: PROFESOR MADYA DR. LIM THEAM SOON

Skill Level: Beginner

General

Announcements

25 October - 29 October

Tuesday Lecture (26 Oct)

Lecture 1 Notes

Lab Report

Recording

Friday Lecture (29 Oct)

Recording Lecture 2

Lecture 2 Notes

1 November - 7 November

Tuesday Lecture (2 Nov)

Lecture 3 Notes

Recording Lecture 3

PCR

Principles of PCR

Friday Lecture (5 Nov)

Lecture 4 Notes

Recording Lecture 4

8 November - 14 November

Tuesday Lecture (9 Nov)

Lecture 5 Notes

Recording Lecture 5

Assignment

PCR-based Assembly Methods

Friday Lecture (12 Nov)

Lecture 6 Notes

Recording Lecture 6

How to choose expression vectors

Expression hosts

15 November - 21 November

Tuesday Lecture (16 Nov 2021)

Lecture 7 Notes

Protein Expression

Recording Lecture 7

Protein Purification Methods

Lab session 1 (18 Nov)

Lab Notes

Lab Report

PCR Primer Design

Tips to design PCR Primers

Friday TEST (19 Nov 2021)

TEST #1

DR. CHEW BEE LYNN

Cloning Vectors

cDNA Libraries

Probes and Genomic Libraries

PRACTICAL 2_RESTRICTION ENDONUCLEASE

SUBMISSION OF PRACTICAL 2

Probes and Southern Blots

Northern and Western Blot

DNA Sequencing

POLYMERASE CHAIN REACTION

PRACTICAL 3 MANUAL

SUBMISSION OF PRACTICAL 3

TEST 2

ASSIGNMENT 2

14 December 2021

Lecture Notes

Lecture Webex

27 December - 2 January

Bioprocessing

Bioreactors

3 January - 9 January

Lab exercise : Measuring Yeast Respiration Rate = f (glucose concentration)

Bioreactor Design and Operations

10 January - 16 January

01 Downstream Processing Overview

02 Separating Cells

03 Cell Disruption

17 January - 23 January

Stirred tank reactor parts and functions

04 Centrifugation

05 Evaporation

06 Liquid Extraction

24 January - 30 January

07 Precipitation

08 Chromatography

BTT306 TECHNIQUES IN BIOTECHNOLOGY